Recombinant vaccinia viruses protect against Clostridium perfringens alpha-toxin

Citation
Am. Bennett et al., Recombinant vaccinia viruses protect against Clostridium perfringens alpha-toxin, VIRAL IMMUN, 12(2), 1999, pp. 97-105
Citations number
31
Categorie Soggetti
Immunology
Journal title
VIRAL IMMUNOLOGY
ISSN journal
08828245 → ACNP
Volume
12
Issue
2
Year of publication
1999
Pages
97 - 105
Database
ISI
SICI code
0882-8245(1999)12:2<97:RVVPAC>2.0.ZU;2-#
Abstract
Recombinant vaccinia viruses that expressed the nontoxic C-domain of Clostr idium perfringens alpha-toxin were constructed. The J2R (thymidine kinase [ TK] gene) and B13R (serpin 2 [SPI-2] gene) loci were used as insertion site s for the clostridial DNA, and expression of the foreign protein was measur ed in each case. A double recombinant that encoded the alpha-toxin truncate at the B13R locus and the protective antigen of Bacillus anthracis at the J2R locus was also constructed. Although differences in expression of the a lpha-toxin C-domain were recorded, all of the vaccinia recombinants protect ed mice against a lethal challenge with alpha-toxin demonstrating that a re combinant vaccinia virus can be used to provide protection against a toxin challenge that is known to be solely antibody mediated.