Stability and folding studies of two homologous proteins, human stefins A and B

In a shorter Introduction we describe the usual experimental means of how t o study protein stability and folding. Our work on stability of human stefi ns A and B, determined by chemical, heat, and pH denaturation, is described next. We explain the folding mechanism of human stefin B (as determined th us far) and compare it to homologous human stefin A, in particular, the dep endence of folding rates on the concentration of GuHCl. pH denaturation of human stefin B (E.Zerovnik et al., Eur. J. Biochem. 1997, 245, 364-372) and its folding to the native state are described in more detail.