Effects of allitridi on intracellular Ca2+ concentration in isolated rat brain cells

AIM: To study actions of allitridi extracted from garlic on intracellular c alcium in isolated rat brain cells. METHODS: Brain cells were isolated from newborn rat brain with Fura 2-AM measurements of intracellular Ca2+ concen tration ([Ca2+](i)). RESULTS: Allitridi 1-100 mu mol . L-1 concentration-de pendently blacked increases of [Ca2+](i) caused by potassium chloride and s odium glutamate (Glu) with IC50 of 59.7 and 69.9 mu mol . L-1 respectively. Allitridi 100 mu mol . L-1 blocked norepinephrine ( Nor)-induced [ Ca2+](i ) elevation. CONCLUSION: Allitridi is an effective agent for blocking the [ Ca2+](i) increase caused by potassium chloride, Nor and Glu.