A new type of Al2O3 sol-gel derived glucose biosensor is developed. The enz
yme electrode comprises glucose oxidase immobilized in the Al2O3 sol-gel ma
trix on a platinized glassy carbon electrode. Platinum black not only provi
des a big specific surface area but also enhances the adhesion of the sol-g
el film. The operational parameters such as the preparation conditions of A
l2O3 sol-gel, the operating potential of the working electrode, and pH effe
ct of response are tested for optimum analytical performance by using an am
permetric method. Linear calibration for glucose is obtained in the range 4
.00 x 10(-5)-2.19 x 10(-2) mM under the optimized conditions. The biosensor
was stable for at least two weeks. (C) 1999 Elsevier Science B.V. All righ
ts reserved.