Analytical control of pharmaceutical production steps by near infrared reflectance spectroscopy

A method for the analytical control of different pharmaceutical production steps involving various types of sample (blended products, cores and coated tablets) is proposed. The measurements are made by using a near infrared ( NIR) diffuse reflectance spectrophotometer furnished with a fibre-optic mod ule that enables expeditious, flexible analyses with no sample manipulation . Calibration for the active principle in the pharmaceutical is done by apply ing partial least-squares regression to first-derivative spectra over the w avelength range 1100-2200 run. Various spectral pretreatments for minimizin g spectral scattering were tested. All samples studied were analysed by usi ng a single calibration including spectra of laboratory samples in order to expand the concentration range spanned by the production samples, as well as samples obtained in different steps of the production process in order t o include its variability sources (variations in origin of raw materials, p article size distribution, compactness, granulation, etc.). Production samp les for inclusion in the calibration set were chosen by principal component analysis. The partial least squares model based on first-derivative spectr a provided a relative standard error of prediction lower than 1% for produc tion samples. (C) 1999 Elsevier Science B.V. All rights reserved.