Determination of pindone in baits by using time-resolved lanthanide-sensitized luminescence and kinetic methodology

A simple and fast kinetic method for the determination of pindone in baits was developed. It is based on the inhibitory effect of this indanedione rod enticide on the luminescence of the europium(III)-thenoyltrifluoracetone sy stem in the presence of cetyltrimethylammonium bromide. The relatively long lifetime of this system allows the time-resolved mode to be used, which el iminates any fluorescence signal. To avoid the potential static background signal of the sample, the difference between the initial rate obtained in t he absence and presence of pindone is used as the analytical parameter, whi ch is obtained within only 0.2 s with the aid of a stopped-now mixing techn ique. The calibration graph is linear in the range 0.1-10.0 mu g ml(-1) pin done and the detection limit is 0.04 mu g ml(-1). The relative standard dev iation is close to 3%. The recoveries obtained by applying the method direc tly to the analysis of bait samples ranged from 92.0 to 114%.