CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF MOUSE-TUMOR NECROSISFACTOR

Authors
BAEYENS KJ DEBONDT HL RAEYMAEKERS A DERANTER CJ FIERS W
Citation
Kj. Baeyens et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF MOUSE-TUMOR NECROSISFACTOR, Acta crystallographica. Section D, Biological crystallography, 53, 1997, pp. 329-330
Citations number
12
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
53
Year of publication
1997
Part
3
Pages
329 - 330
Database
ISI
SICI code
0907-4449(1997)53:<329:CAPSOM>2.0.ZU;2-G
Abstract
Well diffracting crystals of recombinant mouse tumor necrosis factor ( mTNF) have been obtained. The sitting-drop vapor-diffusion method was used to grow crystals suitable for X-ray studies from solutions contai ning methoxypolyethylene glycol 2000 and isopropanol as precipitants. The crystals belong to the space group P1 with unit-cell dimensions a = 49.40, b = 48.24, c = 51.13 Angstrom, alpha = 115.06, beta = 103.32, gamma = 91.27 degrees and one trimer in the asymmetric unit. Crystals are stable to X-rays and diffract beyond 2.0 Angstrom. Cooling the cr ystal during data collection is necessary since crystals dissolve at r oom temperature.