Ultrastructural and molecular characterization of the parasporal crystal proteins of Bacillus thuringiensis subsp kurstaki S93 active against Spodoptera frugiperda

Citation
Mt. De-souza et al., Ultrastructural and molecular characterization of the parasporal crystal proteins of Bacillus thuringiensis subsp kurstaki S93 active against Spodoptera frugiperda, BIOCELL, 23(1), 1999, pp. 43-49
Citations number
17
Categorie Soggetti
Cell & Developmental Biology
Journal title
BIOCELL
ISSN journal
03279545 → ACNP
Volume
23
Issue
1
Year of publication
1999
Pages
43 - 49
Database
ISI
SICI code
0327-9545(199904)23:1<43:UAMCOT>2.0.ZU;2-R
Abstract
A Brazilian strain of Bacillus thuringiensis subsp. kurstaki (S93), which w as previously shown to be more toxic to Spodoptera frugiperda than the comm ercially available strain HD 1 DIPEL, was characterized. The S93 isolate wa s shown to produce bypiramidal and cuboidal shape crystals. SDS-PAGE of spo rulating cultures showed the presence of mainly two polypeptides of ca. 135 - and 65-kDa, respectively. The presence of these polypeptides was in agree ment with the type of crystals found, cryIA- and cryIIA-type genes were als o detected in the plasmidial DNA from this strains by using PCR. Furthermor e, plasmidial profile of this strain has a very similar content to the HD1 DIPEL strain. These results hint that the Brazilian strain is very similar to the HD1 in several aspects. However, the reasons for the increased activ ity of the Brazilian isolate towards S. frugiperda remain unknown.