In vitro propagation of Philodendrom panduraeforme (Araceae)

A technique for propagation of Philodendron panduraeforme Scott by the cult ure in vitro of axillary buds is described. Shoot regeneration occurred whe n the axillary buds were cultured on Murashige and Skoog (MS) medium lackin g growth regulators. Multiple shoot regeneration was obtained by culturing nodal segments of regenerated shoots on MS medium, solidified with 0.7% aga r and supplemented with 3 mg/L benzyladenine. Root regeneration occurred in high frequency when the regenerated shoots were recultured on strength MS medium, 3% sucrose, 0.7% agar and 2 mg/L activated charcoal. The cultures w ere maintained at a constant temperature of 27 degrees C and a 14 h photope riod (40 mu mol m(-2) s(-1)). The regenerated plantlets were then successfu lly grown in pots.