Neonatal and adult rabbit renal brush border membrane vesicle solute reflection coefficients

Citation
R. Quigley et al., Neonatal and adult rabbit renal brush border membrane vesicle solute reflection coefficients, BIOL NEONAT, 76(2), 1999, pp. 106-113
Citations number
28
Categorie Soggetti
Medical Research General Topics
Journal title
BIOLOGY OF THE NEONATE
ISSN journal
00063126 → ACNP
Volume
76
Issue
2
Year of publication
1999
Pages
106 - 113
Database
ISI
SICI code
0006-3126(199908)76:2<106:NAARRB>2.0.ZU;2-U
Abstract
The interaction between solute and water in epithelial transport is represe nted by the solute reflection coefficient. Because the osmotic water transp ort process changes in the rabbit proximal tubule during maturation, there is a potential for the solute reflection coefficients to also undergo matur ational changes. In the present study, we directly examined solute reflecti on coefficients in neonatal and adult brush border membrane vesicles (BBMV) using the stop-flow lightscattering technique. Reflection coefficients for NaCl, KCl, NaHCO3 and urea were found to be identical in the neonatal and adult BBMV and were not different from 1. Thus, although the water transpor t pathway undergoes changes in the proximal tubule during maturation, there is no evidence for changes in solute and water interaction. Because the re flection coefficients are not different from 1, there is no evidence for so lvent drag in the proximal tubule apical membrane in either the neonatal or adult tubule.