Hot start of the polymerase chain reaction using DNA helicases

A novel method for the hot start of PCR using DNA helicases is developed. T he addition of a DNA helicase prevents the random annealing of primers and synthesis of nonspecific products during the preparation of the reaction mi xture rind initial heating. The hot start of PCR occurs, automatically afte r inactivation of the DNA helicase upon heating of the reaction mixture.