A 3.0-kb DNA fragment containing an endo-inulinase gene was cloned from Art
hrobacter sp. S37. It contained a single open reading frame of 2439 bp, enc
oding a polypeptide composed of signal peptide of 53 amino acids and mature
protein of 759 amino acids. From the comparison with amino acids sequences
of fructan hydrolases and invertase, five highly conserved regions includi
ng the beta-fructosidase motif were found. The sequence of the endo-inulina
se had the identity in the range of 13.3% to 16.0%.