C. Watson et al., Recombinant and tissue extract thromboplastins for determination of international normalised ratio in over-anticoagulated patients, BR J BIOMED, 56(2), 1999, pp. 123-127
The international normalised ratio (INR)/international sensitivity index (I
SI) system is established for calibration of thromboplastins for laboratory
monitoring of oral anticoagulant therapy. The calibration procedure employ
s patients stabilised on oral anticoagulants, and is therefore validated fo
r patients within the therapeutic range. For practical reasons, the system
is used for patients at all levels of therapy, including over-anticoagulate
d patients with particularly low levels of factors II, VII and X. We studie
d patients within and above the therapeutic range, using a thromboplastin c
ontaining recombinant human tissue factor (Innovin) and two tissue extract
thromboplastins. In samples with INRs from 2.0 to 4.0, there was good agree
ment between results obtained with the three systems (mean INRs within 4% o
f each other). In patients with INRs > 4.0, results with a human placental
extract reagent (Thromborel S) were similar to those obtained with a rabbit
brain thromboplastin (IL PT Fib I-Is Plus); mean INRs were 6.30 and 6.32 r
espectively (not significant). Results with Innovin (mean INR: 7.67) were s
ignificantly (P < 0.001) greater (on average by 22%) than those obtained wi
th the other two materials. The discrepancy between results with different
reagents negatively correlated with factor VII levels. Thus, the lower the
factor VII level, the greater was the discrepancy between INRs. Unexpectedl
y, there was a positive correlation between factor V level and the differen
ce between INRs with different reagents. Thus, the higher the factor V leve
l, the greater was the discrepancy between INRs. The effect of these differ
ences at higher INRs on patient management is unknown, but the recently rev
ised UK guidelines recommend that management of these patients should be in
fluenced by clinical factors, reducing the relative importance of discrepan
cies between results obtained with different systems.