Use of allozyme markers to determine genotypes of Phytophthora infestans in Canada

A comparison of allozyme banding patterns at two loci, for glucose-6-phosph ate isomerase (Gpi) and peptidase (Pep), with markers for mating type, meta laxyl sensitivity, and cultural morphology revealed eight distinct genotype s among 726 isolates of Phytophthora infestans collected in Canada between 1994 and 1996. Banding patterns for allozymes of the Gpi locus alone proved to be adequate for distinguishing seven of the genotypes. Five of these ge notypes (US-1, US-6, US-7, US-8, and g11 (or US-II)) were comparable to gen otypes described in the United States. The US-I genotype (Al mating type, m etalaxyl-sensitive) was recovered commonly (41% frequency) throughout Canad a (outside the province of British Columbia) during 1994. By 1996, the US-I genotype was no longer recovered from any samples of potato or tomato tiss ue taken from across Canada. The US-8 genotype (A2 mating type, metalaxyl-i nsensitive) dominated populations of the pathogen in Ontario, Quebec, and N ew Brunswick during 1994 and by 1996, the US-8 genotype was the dominant ge notype in Canada outside British Columbia. In British Columbia, the g11 (US -11) genotype (Al mating type, metalaxyl-insensitive) was the dominant geno type recovered from tissue samples received during 1995 and 1996. Allozyme banding patterns were correlated to mating type, metalaxyl sensitivity, and cultural characteristics, implying that populations were mainly clonal. On ly minor variation in metalaxyl sensitivity was found among provincial popu lations of the US-8 genotype.