Hyperglycaemia abolishes the antihypertrophic efficacy of bradykinin in rat ventricular myocytes

1. Bradykinin inhibits hypertrophy of rat ventricular myocytes, but only in the presence of endothelial cells. 2. The influence of hyperglycaemia on the ability of bradykinin to prevent hypertrophy was investigated in adult rat ventricular myocytes cocultured w ith bovine aortic endothelial cells (BAEC), 3. In myocytes cocultured with normal BAEC, angiotensin II (AngII; 1 mu mol /L) significantly increased [H-3]-phenylalanine incorporation (an in vitro marker of hypertrophy) by 32+/-2%, This was abolished by bradykinin (10 mu mol/L). 4. Pretreatment of BAEC with high glucose (25mmol/L for 24 h) prior to cocu lture with myocytes reduced the antihypertrophic effect of bradykinin, but did not modulate the hypertrophic effect of AngII. 5, Pretreatment of BAEC with hyperglycaemia abolishes the antihypertrophic efficacy of bradykinin in rat ventricular myocytes cocultured with BAEC. Th is has implications for the action of angiotensin-converting enzyme inhibit ors.