Solvent effects on ligninases

The influence of 102 compounds, mostly of solvents, at 0.1 M and 1 M (if so luble) on ligninolytic activity of Phanerochaete chrysosporirrm culture fil trates determined as the rate of veratraldehyde formation has been tested. Most of the compounds, especially the C-1 and cyclic ones, decrease the act ivity of lignin peroxidases. Up to a certain concentration, the decrease ca n be described as inhibition but not as denaturation. Most of the tested pr oton-donating compounds also express intrinsic activity stimulation, wherea s intrinsic activity destimulation is mostly observed among proton accepter s. Where available, the data of 16 solvent property parameters and experime ntal data were analyzed with Principal Component Analysis. The analysis sho wed that no single solvent property parameter correlated well with experime ntal data. The solvents' influence is complex, but hydrogen bonding and ani on stabilization seem particularly important. In the homologous series of c ompounds, the properties represented by the intrinsic molar volume or log P also are important. Some dependencies of the effect of compounds on the st ructures have been established. The solvents suitable for dissolution of co mpounds to be treated with ligninases are suggested. (C) 1999 Elsevier Scie nce Inc. All rights reserved.