Components of the bioemulsifier from S-cerevisiae

The emulsifier isolated by extraction of Saccharomyces cerevisiae biomass w as resolved into two major components. One component contains mainly protei n and some carbohydrate. This appears to be the protein backbone of the pho sphomannoprotein of the yeast cell wall. This component was even more effec tive than the original product obtained from the yeast biomass. Consistent with its properties as an emulsifier, it partitioned strongly to the interf ace between the water and oil phases of an emulsion. The other component co ntained abundant carbohydrate and phosphorous and was attributed to the pho sphomannan fragments from the original cell wall material. It does not appe ar to be an emulsifier, but it enhanced the emulsification properties of th e first component. It was very water soluble, and most of it remained in th e aqueous phase when an emulsion was prepared. (C) 1999 Elsevier Science In c. All rights reserved.