Deletion of a proline-rich region and a transmembrane domain in fatty acidamide hydrolase

Citation
G. Arreaza et Dg. Deutsch, Deletion of a proline-rich region and a transmembrane domain in fatty acidamide hydrolase, FEBS LETTER, 454(1-2), 1999, pp. 57-60
Citations number
24
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
454
Issue
1-2
Year of publication
1999
Pages
57 - 60
Database
ISI
SICI code
0014-5793(19990702)454:1-2<57:DOAPRA>2.0.ZU;2-Q
Abstract
Fatty acid amide hydrolase contains a proline-rich sequence matching a cons ensus sequence for SH3-binding domains as well as a transmembrane domain. I n this study, deletion mutants lacking the proline-rich region and the tran smembrane domain were generated. Transfection experiments demonstrated that the proline-rich deleted amidase was enzymatically inactive. While immunos taining of the wild-type was always punctate with strong perinuclear staini ng characteristic for endoplasmic reticulum, the staining of the mutant was diffuse and distributed throughout the cytoplasm and perinuclear region. T hese observations along with the loss of activity suggest that the proline- rich region may play a role in the subcellular localization and enzymatic f unction. The transmembrane domain-deleted mutant was indistinguishable from the wild-type enzyme. (C) 1999 Federation of European Biochemical Societie s.