Persistence and in vivo effects of Yersinia enterocolitica 0 : 3 endotoxinin rats

In vivo effects of Yeisinia enterocolitica 0:3 lipopolysaccharide (prepared from bacteria grown at 25 degrees C and 37 degrees C) were investigated af ter intraperitoneal (i.p.) and intraarticular (i.a.) injection in rats duri ng 30 days of examination. The persistence of endotoxin in the peritoneal a nd the synovial cavities was demonstrated by the immunofluorescence techniq ue. Peritoneal and synovial exudative cell infiltration, as well as changes in some parameters (glycolytic and acid phosphatase activity, and killing ability of peritoneal cells; lactate dehydrogenase concentration in synovia l fluid) were studied. The results indicated that endotoxin could persist l onger in the synovial than in the peritoneal cavity. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.