A TIME AND COST-EFFECTIVE METHOD FOR KAPPA-CASEIN ALLELE-A AND ALLELE-B GENOTYPING OF BREEDING BULLS

Authors
FLEKNA G ZINOVIEVA N MULLER M BREM G
Citation
G. Flekna et al., A TIME AND COST-EFFECTIVE METHOD FOR KAPPA-CASEIN ALLELE-A AND ALLELE-B GENOTYPING OF BREEDING BULLS, Reproduction in domestic animals, 32(3), 1997, pp. 171-173
Citations number
14
Categorie Soggetti
Reproductive Biology","Veterinary Sciences
Journal title
Reproduction in domestic animals → ACNP
ISSN journal
09366768
Volume
32
Issue
3
Year of publication
1997
Pages
171 - 173
Database
ISI
SICI code
0936-6768(1997)32:3<171:ATACMF>2.0.ZU;2-V
Abstract
The aim of this study was to establish a cost-effective and timesaving method for the routine diagnosis of kappa-casein genotypes of breedin g bulls. DNA of 65 animals was isolated from semen by alkali lysis. Th e genotype was analysed by a single tube allele-specific (STAS) polyme rase chain reaction (PCR). The results showed complete agreement with those of an established method, which is more costly.