The BRCA2 transactivation domain does not interact with JNK

The N-terminal region of BRCA2 has the capacity to activate transcription w hen fused to a heterologous DNA binding domain and includes a segment with amino acid similarity to the JNK-docking site in the cellular JUN protein. However. unlike JUN, we have determined that this region of BRCA2 neither i nteracts with nor serves as a substrate for JNK, or any other kinase that c an be detected in extracts from either fibroblasts or epithelial cells. Whi le this clearly does not rule out a transcriptional role for BRCA2, our fin dings indicate that BRCA2 is not regulated by the JNK pathway in a manner a nalogous to JUN. Genes Chromosomes Cancer 25:407-409, 1999. (C) 1999 Wiley- Liss, Inc.