M. Kirchhoff et al., Deletions below 10 megabasepairs are detected in comparative genomic hybridization by standard reference intervals, GENE CHROM, 25(4), 1999, pp. 410-413
Comparative genomic hybridization (CGH) is a widely used technique for stud
ying chromosomal imbalances. The sensitivity of the technique is, however,
relatively low. Deletions down to a size of 10-12 Mbp have been detected by
the use of fixed diagnostic thresholds. In this study, we applied standard
reference intervals as detection criteria on a number of deletions in the
range of 3 Mbp to 14-18 Mbp. All deletions were detected. Thus, detection b
y standard reference intervals confers a considerably higher sensitivity to
CGH analysis compared to fixed diagnostic thresholds, Genes Chromosomes Ca
ncer 25:410-413, 1999. (C) 1999 Wiley-Liss, Inc.