Procoagulant and anticoagulant activities of resident and inflammatory peritoneal cells

Objective and Design: In the present study, the involvement of both procoag ulants and anticoagulants from adherent peritoneal cells in an inflammatory model was investigated. Material and Treatment: Mice were injected with thioglycollate broth or lip opolysaccharide (LPS). Methods: Cells were harvested by peritoneal lavage. Adherent peritoneal cel ls were cultured further +/- LPS. Subsequently, their ability to activate e ither Factor X or prothrombin, and their ability to inactivate thrombin thr ough a thrombin-degrading mast cell chymase, was assayed. Results: Inflammatory cells expressed reduced amounts of thrombin-inactivat ing activity as compared with control resident peritoneal cells. Both resid ent and inflammatory cells expressed potent prothrombinase activities. Furt her stimulation of the various cellular populations with LPS in vitro had v ery little effect on the prothrombinase and thrombin-inactivating activitie s, bud had a strong stimulatory effect on the Factor X-activating activitie s.