Labeled carcinoembryonic antigen antibodies excitable by infrared rays: A novel diagnostic method for micro cancers in the digestive tract

Object An indocyanine green derivative (ICG-sulfo-OSu) was used as the labe ling substance for monoclonal antibody, and a fluorescence imaging system a ppropriate for ICG-sulfo-OSu excitable by infrared rays (IR) was developed. The goal of this study was to demonstrate antibody labeling at the tissue level using this new imaging system. Materials and Methods ICG-sulfo-OSu la beled mouse anti-human carcinoembryonic antigen (CEA) monoclonal antibody, a newly developed imaging system, and an infrared ray microscope were emplo yed in this experiment. Paraffin sections of human colon cancer previously proven to have cross-reactivity to anti-CEA antibody were examined. Results Positive staining was seen as a brownish discoloration of oxidized 3,3'-di aminobenzidine tetrahydrochloride (DAB) in sections that reacted with ICG-s ulfo-OSu-labeled anti-CEA antibody, and the fluorescence was well-matched w ith the oxidized DAB-positive sites. Conclusion Specific antibodies labeled with ICG-sulfo-OSu have significant affinity to cancer cells and seem to r eflect sufficient amounts of fluorescence by IR to be useful in a system fo r the endoscopic detection of micro cancers using the immunohistochemical s taining method.