Agrococcus citreus sp nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria)

A bacterial strain, D-1/1a(T), isolated from a medieval wall painting of th e chapel of Herberstein (Styria, Austria) was characterized by a polyphasic approach. Strain D-1/1a(T) shared 98.1 % 16S rRNA sequence similarity to A grococcus jenensis. The chemotaxonomic characteristics including polar lipi d pattern, whole cell sugars, quinone system, polyamine pattern, cell wall composition and fatty acid profile were in good agreement with those of Agr ococcus jenensis. The G + C content of the DNA was determined to be 74 mol% . The value of 47 % DMA reassociation obtained after DNA-DNA hybridization between DNA of Agrococcus jenensis and strain D-1/1a(T) as well as differen ces in the amino acid composition of the peptidoglycan and in physiological characteristics demonstrate that the isolate represents a new species of t he genus Agrococcus. The name Agrococcus citreus sp. nov. is proposed for t he new species harbouring isolate D-1/1a(T). The type strain is DSM 12453(T ).