M. Wieser et al., Agrococcus citreus sp nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria), INT J SY B, 49, 1999, pp. 1165-1170
A bacterial strain, D-1/1a(T), isolated from a medieval wall painting of th
e chapel of Herberstein (Styria, Austria) was characterized by a polyphasic
approach. Strain D-1/1a(T) shared 98.1 % 16S rRNA sequence similarity to A
grococcus jenensis. The chemotaxonomic characteristics including polar lipi
d pattern, whole cell sugars, quinone system, polyamine pattern, cell wall
composition and fatty acid profile were in good agreement with those of Agr
ococcus jenensis. The G + C content of the DNA was determined to be 74 mol%
. The value of 47 % DMA reassociation obtained after DNA-DNA hybridization
between DNA of Agrococcus jenensis and strain D-1/1a(T) as well as differen
ces in the amino acid composition of the peptidoglycan and in physiological
characteristics demonstrate that the isolate represents a new species of t
he genus Agrococcus. The name Agrococcus citreus sp. nov. is proposed for t
he new species harbouring isolate D-1/1a(T). The type strain is DSM 12453(T
).