Phenotypic and phylogenetic studies were performed on an unknown Grampositi
ve catalase-negative coccus isolated from a common otter (Lutra lutra). Com
parative 16S rRNA gene sequencing demonstrated that the unknown bacterium r
epresents a new subline within the genus Vagococcus. close to, but distinct
from, Vagococcus fluvialis and Vagococcus salmoninarum. The unknown bacter
ium was readily distinguished from the two currently recognized Vagococcus
species by biochemical tests and electrophoretic analysis of whole-cell pro
teins. Based on phylogenetic and phenotypic evidence it is proposed that th
e unknown bacterium be classified as a new species, Vagococcus lutrae, the
type strain of which is CCUG 39187(T).