Comparison of the capability limits of some optical detectors for liquid chromatography

The sensitivity limits of optical detectors for liquid chromatography are c ompared. The detectors were based on effects related to light absorption (a bsorption, fluorimetric, and photothermal detectors). The lowest detectable optical absorption coefficient of an analyte was used as a criterion for t he limit of detection. Thus, it was unnecessary to consider particular subs tances, procedures, and separation conditions. Choosing the most sensitive detector depends on the power of the light source, all other factors being equal. Starting from powers of several microwatts, preference should be giv en to a fluorimetric or photothermal detector, depending on the quantum yie ld of luminescence.