Purification and molecular characterization of ortho-chlorophenol reductive dehalogenase, a key enzyme of halorespiration in Desulfitobacterium dehalogenans

ortho-Chlorophenol reductive dehalogenase of the halorespiring Gram-positiv e Desulfitobacterium dehalogenans was purified 90-fold to apparent homogene ity, The purified dehalogenase catalyzed the reductive removal of a halogen atom from the ortho position of 3-chloro-4-hydroxyphenylacetate, a-chlorop henol, 2,3-dichloropenol, 2,4-dichlorophenol, 2,6-dichlorophenol, pentachlo rophenol, and 2-bromo-4-chlorophenol with reduced methyl viologen as electr on donor. The dechlorination of 3-chloro-4-hydroxyphenylacetate was catalyz ed by the enzyme at a V-max of 28 units/mg protein and a K-m of 20 mu M. Th e pH and temperature optimum were 8.2 and 52 degrees C, respectively. EPR a nalysis indicated one [4Fe-4S] cluster (midpoint redox potential (E-m) = -4 40 mV), one [3Fe-4S] cluster (E-m = +70 mV), and one cobalamin per 48-kDa m onomer, The Co(I)/Co(II) transition had an E-m of -370 mV, Via a reversed g enetic approach based on the N-terminal sequence, the corresponding gene wa s isolated from a D, dehalogenans genomic library, cloned, and sequenced. T his revealed the presence of two closely linked genes: (i) cprA, encoding t he o-chlorophenol reductive dehalogenase, which contains a twin-arginine ty pe signal sequence that is processed in the purified enzyme; (ii) cprB, cod ing for an integral membrane protein that could act as a membrane anchor of the dehalogenase, This first biochemical and molecular characterization of a chlorophenol reductive dehalogenase has revealed structural resemblance with haloalkene reductive dehalogenases.