New approach to the computer-assisted selection of mobile phases for high-performance liquid chromatography on the basis of the Snyder theory

A new approach, based on the Snyder theory, consisting of the computer-assi sted selection of suitable mobile phases for separation of non-ionic organi c compounds by normal-phase liquid-solid chromatography is detailed. The ad sorption properties of the mobile phases and the chromatographed compounds are predicted by taking into account literature data for the composing solv ents and functional groups, respectively. The approach was successfully app lied to the separation of twenty cis and trans eaters by high-performance l iquid chromatography (HPLC) using a preliminary characterized adsorbent and three suitable computer-selected mobile phases. (C) 1999 Elsevier Science B.V. All rights reserved.