Comparison of the responses of triacylglycerols with an evaporative visible light scattering detector used in conventional, micro and capillary liquid chromatography

During recent years, there has been a growing interest in the development o f packed nano- and microchromatographies. It needs coupling the systems use d to detectors adapted for microchromatographies. In the reported results, the properties of two different nebulizers of an evaporative visible Light scattering detector have been studied under different flow-rate conditions (conventional HPLC, micro-LC and capillary LC). In micro-LC and capillary L C, it is shown that the calibration curves gained for triacylglycerols anal yzed in non-aqueous reversed-phase liquid chromatography could be statistic ally assimilated to a linear relationship even if they rigorously follow a well-known power relationship such as area = a.mass(b). This makes the esta blishment of the calibration curves easier in miniaturized chromatographies than in conventional ones. Depending on the chromatography used, the linea r range falls between either 2-50 ng or 14-80 ng. The limit of quantificati on was 5 ng (S/N = 10). On the other hand, a study of the response of modif ied nebulizer versus flow-rate has shown that the modified evaporative visi ble light scattering detector is more complex than detectors whose response is really proportional to the concentration or to the mass. (C) 1999 Elsev ier Science B.V. All rights reserved.