Determination of the geometrical isomers of ethyl 2,4-decadienoate

Ethyl 2,4-decadienoate isomers, important aroma compounds in the Bartlett p ear, were investigated. Separation and identification of isomers was pet-fo rmed. Separation of isomers was successful using gas chromatography (GC) wi th a VOCOL capillary column and high-performance liquid chromatography on a nonpolar reversed-phase column (C-18) with silver ions in mobile phase. Id entification of the isomers was made by comparison of their retention times with the authentic ethyl trans-2,cis-4-decadienoate standard, and by NMR s pectrometry. For quantitative determination of ethyl 2,4-decadienoate isome rs the limit of detection and the interval of linearity for ethyl trans-2,c is-4-decadienoate were determined for both chromatographic methods. (C) 199 9 Elsevier Science B.V. All rights reserved.