EFFECT OF ACETALDEHYDE GENERATED FROM ETHANOL BY ADH-TRANSFECTED CHO CELLS ON THEIR MEMBRANE FATTY-ACID PROFILES

Ethanol has been previously shown to reduce the unsaturated fatty acid content of cell membranes. It is not known, however, if the observed deleterious effects are due to ethanol itself or its metabolite, aceta ldehyde. The present study was undertaken to assess the effect of acet aldehyde produced from ethanol by alcohol-dehydrogenase-transfected Ch inese hamster ovary Cells on the membrane lipids and the lipid peroxid ation measured by free and bound malondialdehyde (MDA). The effects of ethanol alone was assessed in the presence of 4-methylpyrazole (4-MP) , an inhibitor of alcohol dehydrogenase. After 8 days of incubation, t otal cellular lipids were extracted, subjected to TLC, and analyzed by gas chromatography. MDA concentration were determined by thiobarbitur ic acid reaction followed by HPLC detection. The level of acetaldehyde in the culture medium increased with concentration of ethanol from 5 to 20 mM as did the lipid peroxidation. Total cholesterol, phospholipi ds, and triglycerids all increased with increasing concentration of ac etaldehyde. These effects were due to acetaldehyde as they were blocke d by 4-MP. Some changes in fatty acid profiles were observed by effect of ethanol itself. Copyright (C) 1996 Elsevier Science Inc.