Immunoblotting technique for the detection of allergens of Aspergillus fumigatus: Influence of Nonidet P-40 on the sensitivity

Authors
Wijnands, LM Deisz, WDC van Leusden, FM
Citation
Lm. Wijnands et al., Immunoblotting technique for the detection of allergens of Aspergillus fumigatus: Influence of Nonidet P-40 on the sensitivity, J INVES ALL, 9(3), 1999, pp. 195-196
Citations number
5
Categorie Soggetti
Clinical Immunolgy & Infectious Disease
Journal title
JOURNAL OF INVESTIGATIONAL ALLERGOLOGY & CLINICAL IMMUNOLOGY
ISSN journal
10189068 → ACNP
Volume
9
Issue
3
Year of publication
1999
Pages
195 - 196
Database
ISI
SICI code
1018-9068(199905/06)9:3<195:ITFTDO>2.0.ZU;2-B
Abstract
Immunoblotting provides a useful technique for the study of antigens, antib odies and allergens. To overcome problems regarding the loss of antigenic p roperties during the blotting and developing procedures, several solutions have been described. The inclusion of Nonidet P-40, recommended to increase the sensitivity of developing procedures for immunoblots, in an existing p rocedure for the detection of allergens of Aspergillus fumigatus, however, led to decreased sensitivity of the method.