Chiral stationary phases based on cellulose and amylose tris-3,5-dimethylphenylcarbamate derivatives for the resolution of selected chiral drugs and metabolites

Chiral stationary phases based on tris-3,5-dimethylphenylcarbamate derivati ves of cellulose and amylose were used for the resolution of some chiral dr ugs and their metabolites. The Chiralcel OD-H and Chiralpak AD columns were employed under normal phase conditions using hexane/ethanol or hexane/2-pr opanol as the mobile phases. The influence of trace amounts of diethylamine in the mobile phase was also evaluated. The Chiralcel OD-R column was empl oyed under reversed phase condition using mainly perchlorate solution suppl emented with acetonitrile. The results showed that the resolution obtained under normal phase conditio ns in general is better than that obtained using the same kind of stationar y phase under reversed phase conditions. The Chiralpak AD provided the best resolution for all drugs studied.