The effect of CAT trinucleotide interruptions on the age at onset of spinocerebellar ataxia type 1 (SCA1)

The effect of CAT trinucleotide interruptions in the CAG trinucleotide repe ats of the SCA1 gene on the age at onset of spinocerebellar ataxia type 1 ( SCA1) was investigated. The number of CAG repeats in SCA1 was determined by polymerase chain reaction (PCR) analysis, and the presence of CAT interrup tions was assessed on the basis of the sensitivity of the PCR products to t he restriction endonuclease SfaNI, which recognises CAT trinucleotides. Onl y one in 17 expanded SCA1 alleles from 17 SCA1 patients was interrupted by CAT. The SfaNI sensitive SCA1 allele from this single patient contained 58 CAG repeats, which would predict an age at onset of SCA1 of 22.0 years, in contrast to the actual 50 years. In addition, the brain stem atrophy of thi s patient was mild compared with that of a patient with 52 uninterrupted CA G repeats. A sequence analysis showed that the repeat portion of the patien t contained (CAG)(45)CATCAG CAT(CAG)(10). From these results, we suggest th at the age at onset of SCA1 is not determined by the total number of CAG re peats (58) but by the number of uninterrupted CAG repeats.