Acetylcholinesterase activity, and neurofilament protein, and catecholamine synthesizing enzymes immunoreactivities in the mouse adrenal gland duringpostnatal development

The present study showed the acetylcholinesterase (AChE) activity, and neur ofilament protein (NFP), catecholamine-synthesizing enzymes, dopamine beta- hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) immunor eactivities in the mouse adrenal gland during postnatal development. From b irth to postnatal-1-day, AChE activity was weakly and diffusely found in so me medullary cells and in very few nerve fibers whereas strong NFP immunore activity was seen in a few ganglion cells and in remarkably numerous nerve fibers in the medulla. Almost all meduallary cells were reactive for both D BH and PNMT during this period. From postnatal-2- or -3-day to postnatal-1- week, strong AChE activity was observed in a few large ganglion cells, but the reaction was weak in clusters of chromaffin cells, and the number of st rong AChE-active nerve fibers in the medulla was rapidly increased. From po stnatal-2-day onwards, the number of NFP-immunoreactive nerve fibers in the medulla were remarkably numerous. Numerous chromaffin cells were reactive for both DBH and PNMT whereas some chromaffin cells were reactive for only DBH from postnatal-2-day onwards. These results suggest that drastic change s such as an increase of acetylcholine in the nerve fibers, differentiation of noradrenaline and adrenaline cells of the medulla may occur during this period. From postnatal-2-week to postnatal-3-week, weak AChE activity was seen in the culsters of several chromaffin cells and a few ganglion cells, and the number of AChE-active nerve fibers in the medulla was gradually inc reased. From postnatal-4-week to postnatal-8-week (adult), the distribution and frequency of AChE activity in the adrenal gland were similar to those at postnatal-3-week. In the adult, AChE activity was weakly seen in the clu sters of several chromaffin cells showing noradrenaline fluorescence in the adrenal medulla. The noradrenaline cells were contacted by denser AChE-rea ctive nerve fibers than adrenaline cells. These results suggest that the de velopment of cholinergic nervous system in the mouse adrenal medulla may be completed by postnatal-3-week.