Characterization of feline CD56 molecule expressed on insect cells by the baculovirus expression system

Authors
Nishimura, Y Miyazawa, T Ikeda, Y Izumiya, Y Nakamura, K Cai, JS Sato, E Kohmoto, M Mikami, T Takahashi, E
Citation
Y. Nishimura et al., Characterization of feline CD56 molecule expressed on insect cells by the baculovirus expression system, J VET MED S, 61(6), 1999, pp. 701-703
Citations number
7
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF VETERINARY MEDICAL SCIENCE
ISSN journal
09167250 → ACNP
Volume
61
Issue
6
Year of publication
1999
Pages
701 - 703
Database
ISI
SICI code
0916-7250(199906)61:6<701:COFCME>2.0.ZU;2-0
Abstract
Recently we cloned 140 kDa form of feline CD56 cDNA. In this study, we expr essed the feline CD56 molecule by the baculovirus expression system. We fou nd that the molecule was expressed on the cell surface when examined by the indirect immunofluorescence assay using an anti-human CD56 monoclonal anti body. Immunoblotting analysis revealed that the molecular weight of the maj or expressed product was 140 kDa. Interestingly we found that the insect ce lls expressing the feline CD56 molecule aggregated, indicating that the exp ressed molecule mediates hemophilic adhesion.