Rjg. Mortimer et al., Use of gel probes for the determination of high resolution solute distributions in marine and estuarine pore waters, MAR CHEM, 63(1-2), 1998, pp. 119-129
Pare water profiles were obtained at high resolution (millimeter scale) in
marine sediments using a DET (diffusive equilibration in thin films) gel pr
obe. A plastic probe which holds a 1-2 mm thick polyacrylamide gel covered
by a 0.45 mu m Millipore filter is inserted into the sediment. The gel was
prehydrated in water of similar salinity to the in situ sampling conditions
. Chloride and sulphate needed 24 and 48 h for complete front and back equi
libration, respectively, while 6-8 h (front) and 4-6 h (back) were used for
calcium and alkalinity. Ammonia-N and total CO2 were back-equilibrated for
2 h. Probes were sectioned immediately after sampling, stored for up to 1-
2 days (NH4+, Sigma CO2) or for up to 1-2 weeks (Ca, alkalinity, Cl, SO4, N
O3), before they were back-equilibrated into Milli-Q water or 0.7 hi NaCl (
calcium and alkalinity). The gel retains between 3-7% of the total sulphate
. A simple procedure has been developed to correct for this incomplete reco
very. Recovery tests using seawater and 50% seawater spiked to concentratio
ns found in nearshore pore waters, showed recoveries of 101.4 +/- 0.5% (chl
oride), 101.4 +/- 0.7% (bromide), 100.3 +/- 0.2% (nitrate), 96.6 +/- 0.7% (
sulphate), 99.7 +/- 0.8% (ammonia-N), 99.1 +/- 1.2% (Sigma CO2), 96.9 +/- 0
.8% (calcium) and 96.8 +/- 1.4% (alkalinity) (mean +/- standard error). Por
e water profiles obtained simultaneously using gel probes and conventional
techniques (box core-anoxic slicing followed by centrifugation) showed exce
llent comparability at cm resolution though features which required higher
resolution which could only be seen in the gel profiles. (C) 1998 Elsevier
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