Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis

Citation
Ae. Montoya et al., Electrophoretic karyotype of environmental isolates of Paracoccidioides brasiliensis, MED MYCOL, 37(3), 1999, pp. 219-222
Citations number
17
Categorie Soggetti
Microbiology
Journal title
MEDICAL MYCOLOGY
ISSN journal
13693786 → ACNP
Volume
37
Issue
3
Year of publication
1999
Pages
219 - 222
Database
ISI
SICI code
1369-3786(199906)37:3<219:EKOEIO>2.0.ZU;2-1
Abstract
Five of the 12 environmental isolates of the dimorphic fungus Paracoccidioi des brasiliensis known to date, were analysed by contour-clamped homogeneou s electric field gel electrophoresis (CHEF). The electrophoretic pattern wa s shown to consist of five bands, with molecular size ranging from 3.2 to 1 0 Mb, a model quite similar to the one found in the clinical isolates previ ously tested and used here as controls. However, one of the bands in the en vironmental isolates had a lesser weight (7.2 Mb), than the one correspondi ng to the clinical counterparts (8.8 Mb). This resulted in a smaller genome , approximately 29.7 Mb. The small differences that were found indicate the presence of chromosome polymorphism in this fungus.