Diversity of microorganisms isolated from amber

Claims that organisms can be cultured from amber, if substantiated, would b e significant contributions to our understanding of the evolution, tenacity . and potential spread of life. Three reports on the isolation of organisms from amber have been published. Cano and Borucki recently reported the iso lation of Bacillus sphaericus and Lambert et al. have described a new speci es designated Staphylococcus succinus from 25-40 million year old Dominican amber. These characterized organisms were phylogenetically distant from ex tant relatives and the Staphylococcus sp. sufficiently far removed from oth er extant staphylococci to be considered a new species. Here we report the culture of bacteria from Dominican and previously untested 120 million par old Israeli (Lebanese lode) amber. Twenty-seven isolates from the amber mat rix have been characterized by fatty-acid profiles (FAME) and/or 16S rRNA s equencing. We also performed a terminal restriction fragment pattern (TRF) analysis of the original amber before prolonged culture by consensus primer amplification of the 16S rRNA followed by restriction enzyme digestion of the amplicons. Sample TRFs were consistent with a sparse bacterial assembla ge and included at least five of the isolated organisms. Finally, we micros copically mapped the internal topography of an amber slice.