Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing

Citation
Wh. Mcdonald et al., Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing, MOL CELL B, 19(8), 1999, pp. 5352-5362
Citations number
83
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR AND CELLULAR BIOLOGY
ISSN journal
02707306 → ACNP
Volume
19
Issue
8
Year of publication
1999
Pages
5352 - 5362
Database
ISI
SICI code
0270-7306(199908)19:8<5352:MFYCIA>2.0.ZU;2-B
Abstract
Myb-related cdc5p is required for G(2)/M progression in the yeast Schizosac charomyces pombe. We report here that all detectable cdc5p is stably associ ated with a multiprotein 40S complex. Immunoaffinity purification has allow ed the identification of 10 cwf (complexed with cdc5p) proteins. Two (cwf6p and cwf10p) are members of the U5 snRNP; one (cwf9p) is a core snRNP prote in. cwf8p is the apparent ortholog of the Saccharomyces cerevisiae splicing factor Prp19p. cwf1(+) is allelic to the prp5(+) gene defined by the S. po mbe splicing mutant, prp5-1, and there is a strong negative genetic interac tion between cdc5-120 and prp5-1. Five cwfs have not been recognized previo usly as important for either pre-mRNA splicing or cell cycle control. Furth er characterization of cwf1p, cwf2p, cwf3p, and cwf4p demonstrates that the y are encoded by essential genes, cosediment with cdc5p at 40S, and coimmun oprecipitate with cdc5p, We further show that cdc5p associates with the U2, U5, and U6 snRNAs and that cells lacking cdc5(+) function are defective in pre-mRNA splicing. These data raise the possibility that the cdc5p complex is an intermediate in the assembly or disassembly of an active S. pombe sp liceosome.