Using the beta/alpha peak-height ratio of ATP in P-31 NMR spectra to measure free [Mg2+]: theoretical and practical problems

Authors
Mulquiney, PJ Kuchel, PW
Citation
Pj. Mulquiney et Pw. Kuchel, Using the beta/alpha peak-height ratio of ATP in P-31 NMR spectra to measure free [Mg2+]: theoretical and practical problems, NMR BIOMED, 12(4), 1999, pp. 217-220
Citations number
15
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
NMR IN BIOMEDICINE
ISSN journal
09523480 → ACNP
Volume
12
Issue
4
Year of publication
1999
Pages
217 - 220
Database
ISI
SICI code
0952-3480(199906)12:4<217:UTBPRO>2.0.ZU;2-T
Abstract
A recently described alternative method for determining free [Mg2+] in cell s and tissue with P-31 NMR spectroscopy, which is based on the peak-height ratio of the beta and alpha resonances of ATP (h(beta/alpha)) [J. Biol. Che m. 271, 21141-21150 (1996)], was found to have numerous problems. These pro blems include a flaw in the basic multiple equilibrium analysis, the inappl icability of the calibration curve to in vivo studies, and a lack of sensit ivity of the measured ratio to changes in free [Mg2+] at 'clinical' field s trengths. This suggests that the use of this procedure will be very limited . Copyright (C) 1999 John Wiley & Sons, Ltd.