Binding of substrates by purine nucleoside phosphorylase (PNP) from Cellulomonas sp. - Kinetic and spectrofluorimetric studies.

Authors
Wielgus-Kutrowska, B Tebbe, J Wierzchowski, J Shugar, D Saenger, W Koellner, G Bzowska, A
Citation
B. Wielgus-kutrowska et al., Binding of substrates by purine nucleoside phosphorylase (PNP) from Cellulomonas sp. - Kinetic and spectrofluorimetric studies., NUCLEOS NUC, 18(4-5), 1999, pp. 871-872
Citations number
5
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
4-5
Year of publication
1999
Pages
871 - 872
Database
ISI
SICI code
0732-8311(1999)18:4-5<871:BOSBPN>2.0.ZU;2-P
Abstract
Dissociation constants and stoichiometry of binding for interaction of Cell ulomonas sp. purine nucleoside phosphorylase with its substrates: inosine/g uanosine, orthophosphate, guanine/hypoxanthine and D-ribose-1-phosphate wer e studied by kinetic and spectrofluorimetric methods.