In vitro influence of parenteral lipid emulsions on the respiratory burst of neutrophils

The in vitro effect of a fish oil-derived lipid emulsion (omega-3) on the s uperoxide anion production during the respiratory burst (RB) of human neutr ophils was compared to a LCT lipid (Intralipid), and an LCT/MCT emulsion (L ipofundin MCT). The effects of two concentrations (60 and 600 mu g/mL) were evaluated by rhodamine in a flow cytometer. The RE was induced either by s timulation with Escherichia coli (E. coli) or by priming with TNF-alpha and FMLP stimulation. The results (mean +/- SD%, P < 0.05) were compared to po sitive control responses (RB without lipids). omega-3 (60 mu g/mL, -8.2 [9. 3]%; 600 mu g/mL, -9.6 [11.1]%) and LCT (600 mu g/mL, -8.0 [9.3]%) signific antly suppressed the RE after stimulation with E, coli. LCT/MCT increased t he RE after E. coli (60 mu g/mL, 15.7 [15.4]%; 600 mu g/mL, 42.7 [21.4]%) a s well as after TNF-alpha/FMLP stimulation (600 mu g/mL, 27.4 [23.7]%). The in vitro influence of parenteral lipid emulsions on the superoxide anion p roduction of human neutrophils is dependent on the length of the fatty acid molecule. (C) Elsevier Science Inc.