Identification of chiro-inositol-containing phospholipids and changes in their metabolism upon salt stress in soybean seedlings

We report here the presence of chiro-inositol-containing phospholipids in a ddition to the common myo-inositol-containing phospholipids in soybean seed lings. Using sequential TLC and HPTLC, eleven inositol phospholipids were i solated from soybean seedlings and analyzed after 6 N HCl hydrolysis for th eir inositol and fatty acid compositions by anion exchange Dionex HPLC and CC, respectively. Upon analysis, we have identified three species of chiro- inositol-containing phospholipids and further observed that the chiro-inosi tol-containing phospholipids and their fatty acid composition changed in re sponse to 1 and 24 h 150 mM NaCl treatment compared to the control (no NaCl treatment). In addition, we found the different tissue distribution and me tabolic changes of the chiro-inositol-containing phospholipids in apical, e longating and mature parts of hypocotyls and roots of soybean seedlings upo n salt stress. (C) 1999 Elsevier Science Ltd. All rights reserved.