A new furostanol glycoside was isolated from the rhizomes of Costu spicatus
. Its structure was established as (3 beta,22 alpha,25R)-26(beta-D-glucopyr
anosyloxy)-22-methxyfurost-5-en-3yl O-beta-D-furanosyl-(1 --> 2)-O-[6-deoxy
-alpha-L-mannopyranosyl-(1 --> 4)]-beta-D-glucopyranoside. The structural i
dentification was performed using detailed analysis of H-1 and C-13 NMR spe
ctra including 2D NMR spectroscopic techniques (COSY, HETCOR and COLOC) and
chemical conversions. (C) 1999 Elsevier Science Ltd. All rights reserved.