The in situ spatial arrangement of the influenza A virus matrix protein M1assessed by tritium bombardment

Authors
Shishkov, AV Goldanskii, VI Baratova, LA Fedorova, NV Ksenofontov, AL Zhirnov, OP Galkin, AV
Citation
Av. Shishkov et al., The in situ spatial arrangement of the influenza A virus matrix protein M1assessed by tritium bombardment, P NAS US, 96(14), 1999, pp. 7827-7830
Citations number
24
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
00278424 → ACNP
Volume
96
Issue
14
Year of publication
1999
Pages
7827 - 7830
Database
ISI
SICI code
0027-8424(19990706)96:14<7827:TISSAO>2.0.ZU;2-F
Abstract
Intact influenza A virions were bombarded with thermally activated tritium atoms, and the intramolecular distribution of the label in the matrix prote in M1 was analyzed to determine the in situ accessibility of its tryptic fr agments. These data were combined with the previously reported x-ray crysta l structure of the M1 fragment 2-158 [Sha, B, & Luo, M. (1997) Nat. Struct. Biol, 4, 239-244] and the predicted topology of the C domain (159-252) to propose a model of M1 arrangement in the virus particle.