A modified procedure for fast purification of T7 RNA polymerase

The in vitro T7 transcription system allows one to synthesize biochemical a mounts of RNA molecules functionally equivalent or similar to those transcr ipts normally existing at extremely low levels in vivo. In this study we de scribed a modified method for efficient large-scale preparation of pure T7 RNA polymerase free of RNase activity from the recombinant Escherichia coli strain BL21/pAR1219 (4). The procedure, which used preparative column chro matography on DEAE-Sepharose CL-GB and Blue 3GA, was shown to be simple, ra pid, and cost effective in comparison with other methods reported previousl y, (C) 1999 Academic Press.