Antigenic response of midgut (Boophilus microplus) cell culture vaccine

Antigenic response of midgut, Boophilus microplus, cell culture vaccine was evaluated. The antibody titres against the gut-membrane (GM) and gut-solub le (GS) antigens were measured by indirect hemagglutination test. The antib ody response against GM antigen was significantly higher (p < 0.01) than th at of GS antigen. It was assumed on the basis of antibody titres against GM and GS antigens that the in vitro propagated midgut cells, Boophilus micro plus, contained substantial membrane and membrane associated immunogenic co mponents. (C) 1999 Elsevier Science B.V. All rights reserved.