V. Hecht et al., A NEW ARABIDOPSIS NUCLEIC-ACID BINDING-PROTEIN GENE IS HIGHLY EXPRESSED IN DIVIDING CELLS DURING DEVELOPMENT, Plant molecular biology, 34(1), 1997, pp. 119-124
An Arabidopsis thaliana cDNA encoding a new RNA-binding protein (RBP37
) was cloned from a silique cDNA library. The predicted amino acid seq
uence corresponds to a REP containing two RNA recognition motifs (RRM)
and a basic domain. An affinity for nucleic acids was confirmed in bi
nding assays using in vitro synthesised AtRBP37 protein. in situ hybri
disation experiments on sections of flowers and siliques showed expres
sion only in growing organs: gynoecium, petals, filaments and during e
arly-embryogenesis expression is located in the embryo proper and the
suspensor up to late heart stage. Expression is not detected in the em
bryo during maturation. This results suggests an expression pattern co
rrelated with dividing cells.